Translational_Unit
virE1-3

Part:BBa_K5088702:Design

Designed by: Dascha Khalfine   Group: iGEM24_Marburg   (2024-08-06)


TU virE1-3 from A. tumefaciens TiBo542


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1464
    Illegal XbaI site found at 251
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1464
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1464
    Illegal BglII site found at 1099
    Illegal BglII site found at 3094
    Illegal BglII site found at 3250
    Illegal BamHI site found at 488
    Illegal XhoI site found at 2844
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1464
    Illegal XbaI site found at 251
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1464
    Illegal XbaI site found at 251
    Illegal NgoMIV site found at 1299
    Illegal NgoMIV site found at 2057
    Illegal NgoMIV site found at 3680
    Illegal AgeI site found at 3737
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References